- Jennifer is a playable character for the first time.

- Grady/Greddy. May be a reference to both Grady from The Shining and Geddy Lee of Rush.

- Melton. A reference to The Toxic Avenger.

- Damiyan/Damien. A reference to The Omen.

- Bem. A reference to the horror anime Yokai Ningen Bem.

- Cultist. Soul Drop = Cultist. Item Drop = Extract, Charred Newt.

- Lantern Boy. Similar to the one-eyed Pumpkin Kids from Wanpaku Graffiti. Soul Drop = Lantern Boy. Item Drop = Necronomicon, Extract.

- The Pumpkin King.

- Zombies. Item Drop = Charred Newt.

- Rashia. Soul Drop = Vegetable. Item Drop = Explash, Stone of Sage.

- Dame. Soul Drop = Dame. Item Drop = Necronomicon, Charred Newt.

- Fang Ant. Item Drop = Extamin.

- Fried Centipede.

- Mothman. Soul Drop = Moth. Item Drop = Charred Newt, Sand of Solomon, Soul Ball.

- Cockroach. Soul Drop = Cockroach.

- Brundle.

- Horned Frog. Soul Drop = Horned Frog. Item Drop = Frog's Liver.

- Poison Frog. Soul Drop = Poison Frog. Item Drop = Mandragora.

- Needle Lizard. Soul Drop = Needle Lizard. Item Drop = Lizard's Liver.

- Tail Lizard. Item Drop = Extract.

- Rock Snake. Soul Drop = Rock Snake. Item Drop = Mandragora, Extract.

- Snail. Soul Drop = Snail.

- Momangar. Soul Drop = Momangar.

- Rats. Soul Drop = Rats. Item Drop = Extract.

- Big Rat. Soul Drop = Big Rat. Item Drop = Rat's Liver.

- Sleeping Cat. Soul Drop = Sleeping Cat. Item Drop = Rat's Liver.

- Hell Cat. Soul Drop = Hell Cat. Item Drop = Cat's Liver.

- Gujo. Soul Drop = Gujo. Item Drop = Cat's Liver, Charred Newt.

- Kick Crow. Soul Drop = Bird. Item Drop = Sand of Solomon.

- A headless chicken. Previously seen in Wanpaku Graffiti. Soul Drop = Chicken.

- Big Rooster. Soul Drop = Big Rooster. Item Drop = Stone of Sun.

- Knife & Fork. Item Drop = Extract.

- Candle.

- Pierrot. Soul Drop = Van Gogh. Item Drop = Soul Ball.

- Invisible Boy. Soul Drop = Napoleon.

- Sympathizer. Soul Drop = Sympathizer, Necronomicon.

- Maniac. Soul Drop = Hitler. Item Drop = Chainsaw, Stone of Death.

- Meatbag. Item Drop = Explash, Extamin.

- Boody. Soul Drop = Hog. Item Drop = Explash.

- Nuts.

- Gorigan. Soul Drop = Apeman. Item Drop = Stone of Solomon.

- Leonardo. Soul Drop = Lion.

- Mayor.

- Gil-Man. Soul Drop = Gil-Man. Item Drop = Gilgamesh, Extamin.

- Wart. Soul Drop = Fish. Item Drop = Gilgamesh, Extamin.

- Octopus Girl. Soul Drop = Onono Komachi. Item Drop = Soul Ball.

- Snake Woman. Soul Drop = Snake. Item Drop = Soul Ball, Rabbit Foot.

- Scylla. Soul Drop = Scylla. Item Drop = Carmilla's Hair, Extamin.

- Vamprella. Soul Drop = Bat. Item Drop = Necronomicon, Charred Newt.

- Labat. Soul Drop = Labat. Item Drop = Charred Newt, Bat Liver.

- Mad Door. Item Drop = Sand of Death.

- Mad Car. Soul Drop = Nero. Item Drop = Stone of Death.

- Mad House. Item Drop = Explash.

- St. Germain.

- Spirit. Soul Drop = Cultist.

- Jado. Soul Drop = Munch. Item Drop = Monkey Paw, Sand of Moon.

- Skeleton. Item Drop = Charred Newt.

- Skullantula. Soul Drop = Skullantula. Item Drop = Sand of Death.

- Mummy. Soul Drop = Cleopatra. Item Drop = Mandragora.

- Assistant. Soul Drop = Woman. Item Drop = Mandragora, Explash, Book of Forbidden.

- Knocker. Soul Drop = Knocker. Item Drop = Cat's Liver.

- Banshee. Soul Drop = Banshee. Item Drop = Mandragora, Extamin.

- Reaper. Soul Drop = Human. Item Drop = Mandragora, Book of Forbidden, Extamin.

- Elena. Soul Drop = Yang Guifei. Item Drop = Soul Ball.

- Devil. Soul Drop = Tivil (Little Devil). Item Drop = Sand of Sage.

- Red Imp. Soul Drop = Red Imp. Item Drop = Gilgamesh, Stone of Sage.

- Kappa. Soul Drop = Merman. Item Drop = Charred Newt.

- Ashitee. Soul Drop = Picasso. Item Drop = Stone of Sun.

- Ramses.

- Slime Man. Item Drop = Explash.

- Tadulhu. Item Drop = Charred Newt.

- Carmilla. Item Drop = Charred Newt, Carmilla's Hair.

- Soldier. Soul Drop = Soldier. Item Drop = Book of Forbidden, Soul Ball, Extract, Stone of Death.

- Commander. Item Drop = Book of Forbidden.

- Defense Bots.

- Combatant. Soul Drop = Pig. Item Drop = Stone of Death, Book of Forbidden.

- Leader. Item Drop = Explash, Book of Forbidden.

- Elite Guard. Item Drop = Explash, Book of Forbidden.

- Cadieu. Soul Drop = Cadieu. Item Drop = Cadieu's Liver, Charred Newt.

- Maggot. Soul Drop = Maggot. Item Drop = Charred Newt.

- Aches. Item Drop = Rabbit Foot, Charred Newt.

- Queen X.

- Bem. Presumably one of many, but not the one that joins the party. Item Drop = Sand of Moon, Charred Newt.

- Grady (boss).

- Jennifer (boss).

- Hell Demon. Item Drop = Book of Forbidden, Charred Newt.

- The three forms of Doctor West.

- Paracelsus. Saves the game, also cures Curse/Poison/Zombie/Body Bag.

- Agrippa. Restores HP/MP.